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Photometry

نورسنجی

Light Transmission Dependence on Concentration

Beer’s Law

molecular absorptivity distribution curve

ALSO See Figure 3-5 Page 81

UV/VIS Usage

In the medical field, it is used for the determination of enzymes, vitamins, hormones, steroids, alkaloids, and barbiturates.

These measurements are used in the diagnosis of diabetes, kidney damage, and myocardial infarction, among other ailments. In the pharmaceutical industry, it can be used to measure the purity of drugs during manufacture and the purity of the final product. For example, aspirin, ibuprofen, and caffeine, common ingredients in pain relief tablets, all absorb in the UV and can be determined easily by spectrophotometry.